SEQUENCING METHODS FOR

SOLUTIONS THAT MATTER

PacBio is a premier life science technology company that is designing, developing, and manufacturing advanced sequencing solutions that enable scientists and clinical researchers to improve their understanding of the genome and ultimately, resolve genetically complex problems.

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Revio long-read system
PacBio Revio System provides affordable, high throughput HiFi sequencing at scale. With a high-density, 25 million ZMW SMRT Cell, up to 4 SMRT Cells in parallel, and 24-hour run times Revio system achieves up to 15X higher throughput compared to the previous generation of PacBio instruments. The Revio system features cutting-edge NVIDIA GPUs that provide rapid turnaround time for base calling and HiFi read generation, keeping pace with the sequencing throughput of the instrument. With Google DeepConsensus on board, Revio is the most accurate PacBio long-read system to date. DeepConsensus uses advanced deep learning techniques to further extend the accuracy and yield of HiFi sequencing.
Standard whole genome sequencing on the Revio system include methylation status and provides insights on small variants, structural variants, repeat expansions, methylation, and haplotype phasing from a single library and sequencing run.
Onso short-read system
The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an extraordinary level of accuracy through its unique sequencing by binding (SBB™) technology. With inherently lower sequencing noise, the Onso system enables researchers to uncover rare variants previously missed and do so at lower sequencing depths.
The accuracy provided by the Onso system will help drive groundbreaking discoveries in areas such as liquid biopsy testing for minimal residual disease (MRD), antibiotic resistance monitoring in microbial communities, off-target analysis in gene editing experiments, and many other “needle-in-a-haystack” applications where the highest level of sensitivity is desired.
Sequel IIe long-read system
The PacBio IIe system provides all scientists with access to high throughput, cost effective, highly accurate long-read sequencing. PacBio Sequel IIe offers single molecule real-time (SMRT), long-read, DNA sequencing technology on at large though flexible scale. This platform is suitable for large scale projects such as de-novo sequencing of large and complex genomes, full length transcriptome analysis large amplicon sequencing, as well as structural varian detection and haplotype phasing.
Nanobind DNA Extraction Kits

Nanobind is a novel magnetic disk covered with a high density of micro and nanostructured silica that can be used for rapid extraction and purification of high-quality DNA and RNA. The high surface area and unique binding mechanism give it an extraordinary binding capacity, allowing isolation of high purity, high molecular weight (HMW) DNA in a microcentrifuge tube format.

Unlike magnetic beads and silica spin columns which shear large DNA, Nanobind disks bind and release DNA without fragmentation, to yield DNA up to megabases in length

Kinnex Kits
Kinnex kits for RNA sequencing are designed to provide customers with cost-effective, end-to-end kitted solutions to support their research. The Kinnex kits concatenates smaller DNA fragments into longer, HiFi-ready libraries. Short-read sequencing has difficulty covering entire transcripts, while long-read sequencing of one transcript would traditionally result in unused sequencing capacity. With Kinnex, customers can concatenate transcripts into long libraries for HiFi sequencing, increasing throughput and making long-read RNA sequencing more cost effective.
HIFI SEQUENCING

HiFi reads are produced using circular consensus sequencing (CCS) mode on PacBio long-read systems. HiFi reads provide base-level resolution with 99.9% single-molecule read accuracy. With HiFi sequencing you get the benefits of short reads and traditional long reads in one easy-to-use technology. HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome

SEQUENCING BY BINDING (SBB)
SBB is an innovative sequencing technology that delivers amazing NGS accuracy. By separating the binding and incorporation steps, optimizing the chemistries for each step, and using native nucleotides, greater signal to noise can be achieved resulting in higher accuracy base calls. Compared to sequencing by synthesis (SBS) utilized by traditional short-read sequencers, SBB chemistry gives ~15x lower error rates at any given cycle so that you can achieve near-perfect accuracy in short-read sequencing without having to sequence at unnecessary depths. SBB sequencing overcomes the limitations of existing short-read sequencing platforms, providing ≥90% of bases at Q40+ accuracy.
NANOBIND

Nanobind is a novel magnetic disk covered with a high density of micro and nanostructured silica that can be used for rapid extraction and purification of high-quality DNA and RNA. Unlike magnetic beads and silica spin columns which shear large DNA, Nanobind disks bind and release DNA without fragmentation, to yield DNA up to megabases in length.

SEQUENCE WITH CONFIDENCE USING PACBIO SEQUENCING SYSTEMS

Revio unleashes the power of HiFi sequencing at scale. Onso delivers near-perfect accuracy so you can break through limits of detection. PacBio is the only sequencing company with both highly accurate short-read and long-read technologies.

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